A NOVEL PIGGYBAC TRANSPOSON INDUCIBLE EXPRESSION SYSTEM IDENTIFIES A ROLE FOR AKT SIGNALLING IN PRIMORDIAL GERM CELL MIGRATION.

A novel piggyBac transposon inducible expression system identifies a role for AKT signalling in primordial germ cell migration.

In this work, we describe a single piggyBac transposon system containing both a tet-activator and a doxycycline-inducible expression cassette.We demonstrate that a gene product can be conditionally expressed from the integrated transposon and a second gene can be simultaneously targeted by a short hairpin RNA contained within the transposon, both i

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The screening of reasonable copper and phosphorus content in weathering steel by a high-throughput experimental method

The effect of coupling of copper and phosphorus in steel on atmospheric corrosion behavior was investigated using high-throughput experimental methods.Wire beam electrodes and plates with discrete distribution of composition gradients were prepared through a selective laser melting method.By combining array electrode scanning tests and EPMA, the el

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Jewish Self-Defense against the Blood Libel in Mid-Nineteenth Century Italy: The Badia Affair and Proceedings of the Castilliero Trial (1855-56)

In 1855, the Badia affair, the sequel to a blood libel against a Jewish businessman in a Veneto town, temporarily put in question Tracksuit Bottoms relations between state, society and the Jewish minority in the Lombardo-Venetian Kingdom.After reconstructing the stages of the episode, the present article analyzes the strategies of response to the c

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